An mRNA degrading complex in Rhodobacter capsulatus.

نویسندگان

  • S Jäger
  • O Fuhrmann
  • C Heck
  • M Hebermehl
  • E Schiltz
  • R Rauhut
  • G Klug
چکیده

An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.

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عنوان ژورنال:
  • Nucleic acids research

دوره 29 22  شماره 

صفحات  -

تاریخ انتشار 2001